Detection of microorganisms with antibacterial activities from different industrial wastes and GC-MS analysis of crude microbial extract

Abstract Many soil microorganisms’ i.e., bacteria and fungi produce secondary metabolites called antibiotics. These are used for the treatment of some bacterial, fungal protozoal diseases humans. There is a need isolation broad spectrum antibiotics from microorganisms due to emergence antibiotic resistance. In present study two producing Klebsiella pneumoniae Bacillus cereus were isolated pharmaceutical poultry feed industry Hattar, Haripur Pakistan. Total 10 waste samples collected different industries (Marble, Ghee, Soap, Mineral, Steel, Poultry Feed, Pharmaceutical, Qarshi, Cosmetic Glass). Thirty-three bacterial strains industrial wastes these ten industries. Fourteen out thirty-three exhibited antimicrobial activities against at least one test microbes considered in this including Escherchia coli, Staphylococcus aureus Salmonella typhi. The by standard serial dilution spread plate technique. Morphological characterization isolates was done Gram staining. Nine fourteen initially identified as B. five K. through biochemical characterization. antibacterial tested well diffusion method. Maximum number based on results primary screening, most potential S9, S19, S20, S22 S23 selected screening. maximum activity E. coli S. recorded isolate S19 i.e zones inhibition 6.5mm 9mm while S20 showed 7.5mm 6mm respectively. Molecular identification carried basis 16S rRNA sequence analysis. Finally, accession LC538271and MT078679. Analysis extract GC-MS indicated presence 8 compounds diverse nature structure. Present suggests that may have bacteria. could be utilized production control microbial growth.